![]() LncRNA KCNQ1OT1-mediated cervical cancer progression by sponging miR-1270 as a ceRNA of LOXL2 through PI3k/Akt pathway. PI80196X3) through Fisher Scientific and other channels. This product is sold as Pierce ECL 2 Substrate (Cat. Circular RNA circHIPK3 is downregulated in diabetic cardiomyopathy and overexpression of circHIPK3 suppresses PTEN to protect cardiomyocytes from high glucose-induced cell apoptosis. Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. Clarity and Clarity Max ECL Substrates are compatible with any HRP conjugate and ideal for all western blotting. Therapeutic exosomal vaccine for enhanced cancer immunotherapy by mediating tumor microenvironment. Long non-coding RNA MIR17HG sponges microRNA-21 to upregulate PTEN and regulate homoharringtonine-based chemoresistance of acute myeloid leukemia cells. Stronger signal than Amersham ECL or Pierce ECL Optimized for x-ray film. N6‑methyladenosine‑induced long non‑coding RNA PVT1 regulates the miR‑27b‑3p/BLM axis to promote prostate cancer progression. The best choice for routine, film-imaged chemiluminescent Western blots. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.Publishing research using ab65623? Please let us know so that we can cite the reference in this datasheet.Īb65623 has been referenced in 110 publications. A name you can can rely on count on the strong quality assurance, technical support and reputation behind Thermo Scientific Pierce Protein Research Products. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. No optimization required switching to Pierce ECL substrate from other entry-level ECL substrates does not require optimization or protocol changes. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. PI32106 Pierce ECL Western Blotting Substrate 500mL kit PI80197 Pierce ECL 2 Western Blotting Substrate Sufficient reagent for 250cm2 of membrane Detection Reagent A: 25mL Detection Reagent B: 0. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. More affordable-high quality and performance at a lower price than other suppliers' similar substrates.More imaging options-X-ray, CCD or laser-based imagers.Longer signal duration-sustained light output for as long as 5 hours.The blot was then re-blocked and reprobed with cyclophilin B antibody and detected with Pierce ECL substrate. ![]() Blots were then stripped with Thermo Scientific Restore PLUS Western Blot Stripping Buffer.
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